试剂耗材
Viromer® BLUE(蓝)、GREEN(绿)siRNA和miRNA转染试剂
Viromer来自德国,为创新型转染试剂而生,且只做转染试剂,只卖转染试剂,因为专业,所以值得信赖!
如果您需要转染的细胞非常难转,多次转染不成功的话,建议尝试Viromers®转染试剂,它是基于病毒融合机制的聚合物,该机制以流感病毒血凝素的融合肽为蓝本;当暴露于低pH时,融合肽失电荷,变为疏水性,进入核膜。推荐用于原代细胞,悬浮细胞,分化中细胞或干细胞等难转细胞。Viromers® Green/Blue均适用于siRNA和miRNA转染,分别适用于不同的细胞系(贴壁细胞或悬浮细胞),均表现出高转染效率。
Viromers®优点:- 避免包涵体吞噬:最大化转染效率并降低背景;
- 零电荷:无需去除血清和抗生素,对细胞温和;
- 无脂质:适用于脂肪代谢活跃的细胞,如:脂肪细胞或肝细胞。
- 稳定:实验结果具有重复性;
- 反向转染:适用于高通量筛选。
使用Viromer®BLUE /GREEN , Lipofectamine转染siRNA 到原代小鼠成纤维细胞中,检测目标基因和GAPDH mRNA的表达
使用Viromer®BLUE /GREEN , Lipofectamine转染siRNA 到cancer stem cells / mammospheres中,检测目标基因和GAPDH mRNA的表达
反向转染表现优越,适用于高通量筛选
Viromer®BLUE转染原代巨噬细胞,在384孔板中,使用Viromer®BLUE转染siRNA targeting EEA1到原代巨噬细胞(从buffy coat PBMC 现分离)中,检测endosomes 水平(siRNA targeting EEA1会导致endosomes 水平降低)
订购详情:
表. Viromer® Blue and Green cell preferences货号 品名 规格 价格 备注 VB-01LB-00 Viromer® BLUE 80 T in 24 well 1000 已验证细胞系
参照表6VB-01LB-01 0.3 ml(500T) 5580 VB-01LB-03 3 x 0.3 ml(1500T) 14160 VG-01LB-00 Viromer® GREEN 80 T in 24 well 1000 VG-01LB-01 0.3 ml(500T) 5580 VG-01LB-03 3 x 0.3 ml(1500T) 14160 Category Cell type Viromer® Cell lines Standards: HeLa, Neuro2A, CHO, A549, HepG2, HEK-293 BLUE &GREEN MCF-7 breast adenocarcinoma BLUE RAW264.7 & NIH3T3 BLUE U87, NCH82 & NCH149 glioblastoma BLUE &GREEN MDA-MB468 &-MB231 breast adenocarcinoma BLUE &GREEN CT26, HT-29, HCT116 colon carcinoma BLUE &GREEN P4E6 prostate carcinoma BLUE Hs746T gastric carcinoma BLUE &GREEN MPC mouse pheochromocytoma cells BLUE &GREEN SH-SY5Y neuroblastoma GREEN MRC-5 lung fibroblasts GREEN TEU-2 immortilizedurothelial cells GREEN C2C12 myoblasts BLUE U2OS osteosarcoma BLUE Suspension cells THP-1 monocytes BLUE &GREEN CHO-K1 suspension GREEN Kasumi-4 & MV4-11 myelomonocytic leukemia cells BLUE Primary & Stem cells Pre-adipocytes (3T3-L1, C3H10T1/2) & adipocytes BLUE Schwann cells from mouse brain BLUE Primary mouse hepatocytes BLUE Primary human macrophages & PBMCs BLUE Primary fibroblasts BLUE &GREEN Primary human skeletal myoblasts BLUE Primary human bladder smooth muscles BLUE HUVECs BLUE Cancer stem cells / 3D-mammospheres BLUE Dental follicle stem cells GREEN Special applications 3D-mammosphere / Co-culture transfection BLUE Disaggregated primary human xenograft tumor cells BLUE